Three-dimensional (3D) printing, a subset of additive manufacturing technologies, has attracted significant attention from researchers for both laboratory-based and on-site prototyping since its widespread adoption. Its adaptability and versatility have made it an essential tool across various disciplines, particularly in biotechnology and bioengineering. While conventional manufacturing methods can offer precise material control and compatibility with biological fluids, they often pose significant challenges, such as high costs and the requirement for large, complex setups. These constraints limit their accessibility for experimental needs of biotechnology and bioengineering. However, 3D printers, with their high adaptability and ability to process a wide range of materials, have proven to be remarkably effective in resolving these challenges. Their capability to create custom parts and structures while maintaining compatibility with biomaterials and fluids has opened new possibilities not only in tissue engineering, drug development, and biomedical device fabrication but also across the broader fields of biotechnology, biochemistry, and related sciences. When examining the basic concept and development timeline of 3D printers, it becomes clear that emerging trends in artificial intelligence, robotics, and digitalization are expected to further accelerate their integration into real-world applications. These ongoing advancements are likely to benefit laboratories and production centers involved in biotechnology by speeding up experiments, paving the way for rapid production and testing, and making complex biofabrication processes more accessible and automated, including in areas like tissue engineering and personalized medicine.